sortase a 5 Search Results


sortase a5 enzyme  (Quantum-Si)
90
Quantum-Si sortase a5 enzyme
A) Overview of the Platinum instrument and the principle of Next-Generation Protein Sequencing. After single peptides are bound to the semiconductor chip, fluorescently tagged amino acid recognizers (six recognizers for 13 amino acids) bind each N-terminal amino acid. After aminopeptidase cleavage, the next amino acid is bound. B) Barcoding construct design includes the protein of interest, followed by an aQinity tag for purification, a short linker, a LysC cleavage site, the peptide barcode, a <t>sortase</t> tag for attachment of a covalent linker for sequencing on Platinum, and an optional His tag for purification. C) Barcoded protein enrichment and barcode sequencing workflow showing the steps going from cell lysate to sequencing.
Sortase A5 Enzyme, supplied by Quantum-Si, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sortase a5 enzyme - by Bioz Stars, 2026-04
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A) Overview of the Platinum instrument and the principle of Next-Generation Protein Sequencing. After single peptides are bound to the semiconductor chip, fluorescently tagged amino acid recognizers (six recognizers for 13 amino acids) bind each N-terminal amino acid. After aminopeptidase cleavage, the next amino acid is bound. B) Barcoding construct design includes the protein of interest, followed by an aQinity tag for purification, a short linker, a LysC cleavage site, the peptide barcode, a sortase tag for attachment of a covalent linker for sequencing on Platinum, and an optional His tag for purification. C) Barcoded protein enrichment and barcode sequencing workflow showing the steps going from cell lysate to sequencing.

Journal: bioRxiv

Article Title: Protein Barcoding and Next-Generation Protein Sequencing for Multiplexed Protein Selection, Analysis, and Tracking

doi: 10.1101/2024.12.31.630920

Figure Lengend Snippet: A) Overview of the Platinum instrument and the principle of Next-Generation Protein Sequencing. After single peptides are bound to the semiconductor chip, fluorescently tagged amino acid recognizers (six recognizers for 13 amino acids) bind each N-terminal amino acid. After aminopeptidase cleavage, the next amino acid is bound. B) Barcoding construct design includes the protein of interest, followed by an aQinity tag for purification, a short linker, a LysC cleavage site, the peptide barcode, a sortase tag for attachment of a covalent linker for sequencing on Platinum, and an optional His tag for purification. C) Barcoded protein enrichment and barcode sequencing workflow showing the steps going from cell lysate to sequencing.

Article Snippet: We then incubate with 100 nM G-linker and 2 uM Sortase A5 enzyme (Quantum-Si, USA) in sortase reaction buffer (50 mM Tris-HCL pH 7.5, 150 mM NaCl, and 5 mM CaCl 2 ) at 37°C for 1 hr on thermomixer at 1000 RPM.

Techniques: Sequencing, Construct, Purification, Protein Enrichment